Phytoestrogens in bovine plasma and milk ? LC-MS/MS analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F10%3A00023841" target="_blank" >RIV/60461373:22330/10:00023841 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Phytoestrogens in bovine plasma and milk ? LC-MS/MS analysis

Popis výsledku v původním jazyce

Phytoestrogens belong to a group of polyphenolic plant metabolites which induce biological responses, based on their structural similarity to 17?-estradiol. In order to investigate the relationship between the levels of these biologically active compounds and beneficial health effects, it is neccesary to quantify accurately their levels in foods and biological fluids. In this study, HPLC-MS/MS method for the determination of isoflavones genistein, daidzein, and estrogenic metabolite-equol in bovine plasma and milk was optimised and validated. The method allowed low limits of detection: 5, 2.5 and 0.5 ng/ml for genistein, daidzein and equol, respectively, thus enabling to determine the effect of phytoestrogen-rich diet on the concentration of isoflavones and the metabolite in biological fluids of cows. The feeding experiment, carried out with four dairy cows, showed that a soy-based diet significantly increased both plasma and milk contents of biologically potent equol, therefore, the l

Název v anglickém jazyce

Phytoestrogens in bovine plasma and milk ? LC-MS/MS analysis

Popis výsledku anglicky

Phytoestrogens belong to a group of polyphenolic plant metabolites which induce biological responses, based on their structural similarity to 17?-estradiol. In order to investigate the relationship between the levels of these biologically active compounds and beneficial health effects, it is neccesary to quantify accurately their levels in foods and biological fluids. In this study, HPLC-MS/MS method for the determination of isoflavones genistein, daidzein, and estrogenic metabolite-equol in bovine plasma and milk was optimised and validated. The method allowed low limits of detection: 5, 2.5 and 0.5 ng/ml for genistein, daidzein and equol, respectively, thus enabling to determine the effect of phytoestrogen-rich diet on the concentration of isoflavones and the metabolite in biological fluids of cows. The feeding experiment, carried out with four dairy cows, showed that a soy-based diet significantly increased both plasma and milk contents of biologically potent equol, therefore, the l

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

<a href="/cs/project/QF3025" target="_blank" >QF3025: Látky s estrogenními účinky v potravních řetězcích, ovlivnění zdraví a užitkovosti hospodářských zvířat a bezpečnosti potravin</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech Journal of Food Sciences

ISSN

1212-1800

e-ISSN

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Svazek periodika

28

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

11

Strana od-do

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Kód UT WoS článku

000282056400003

EID výsledku v databázi Scopus

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