Novel approaches to analysis of 3-chloropropane-1,2-diol esters in vegetable oils

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F12%3A43894724" target="_blank" >RIV/60461373:22330/12:43894724 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00216-012-5732-1" target="_blank" >http://dx.doi.org/10.1007/s00216-012-5732-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-012-5732-1" target="_blank" >10.1007/s00216-012-5732-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Novel approaches to analysis of 3-chloropropane-1,2-diol esters in vegetable oils

Popis výsledku v původním jazyce

A sensitive and accurate method utilizing ultra-high performance liquid chromatography (U-HPLC) coupled to high resolution mass spectrometry based on orbitrap technology (orbitrapMS) for the analysis of nine 3-MCPD diesters in vegetable oils was developed. To remove the interfering triacylglycerols (TAGs) that induce strong matrix effects, a clean-up step on silica gel column was used. The quantitative analysis was performed with the use of deuterium-labelled internal standards. The lowest calibration levels estimated for the respective analytes ranged from 2 to 5 ?g kg-1. Good recovery values (89-120%) and repeatability (RSD 5-9%) were obtained at spiking levels of 2 and 10 mg kg-1. As an alternative, a novel ambient desorption ionization technique, direct analysis in real time (DART), hyphenated with orbitrapMS, was employed for no separation, high-throughput, semi-quantitative screening of 3-MCPD diesters in samples obtained by chromatographic fractionation. Additionally, the levels

Název v anglickém jazyce

Novel approaches to analysis of 3-chloropropane-1,2-diol esters in vegetable oils

Popis výsledku anglicky

A sensitive and accurate method utilizing ultra-high performance liquid chromatography (U-HPLC) coupled to high resolution mass spectrometry based on orbitrap technology (orbitrapMS) for the analysis of nine 3-MCPD diesters in vegetable oils was developed. To remove the interfering triacylglycerols (TAGs) that induce strong matrix effects, a clean-up step on silica gel column was used. The quantitative analysis was performed with the use of deuterium-labelled internal standards. The lowest calibration levels estimated for the respective analytes ranged from 2 to 5 ?g kg-1. Good recovery values (89-120%) and repeatability (RSD 5-9%) were obtained at spiking levels of 2 and 10 mg kg-1. As an alternative, a novel ambient desorption ionization technique, direct analysis in real time (DART), hyphenated with orbitrapMS, was employed for no separation, high-throughput, semi-quantitative screening of 3-MCPD diesters in samples obtained by chromatographic fractionation. Additionally, the levels

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

—

Svazek periodika

402

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

13

Strana od-do

2871-2883

Kód UT WoS článku

000301178000018

EID výsledku v databázi Scopus

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