Antibacterial effect of peptide compound isolated from horseradish (Armoracia rusticana) tissue culture

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F15%3A43900467" target="_blank" >RIV/60461373:22330/15:43900467 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Antibacterial effect of peptide compound isolated from horseradish (Armoracia rusticana) tissue culture

Popis výsledku v původním jazyce

Antimicrobial peptides are an abundant group of proteinaceous compounds widely produced in the plant kingdom. Here, we report the purification and partial characterization of a novel antimicrobial peptide compound from horseradish tissue culture (Armoracia rusticana), named Ar-AMP, with bactericidal activity against both Gram-positive and Gram-negative bacteria. The production of antimicrobial peptides was induced with fungi Aspergillus ochraceus. Ar-AMP was isolated by precipitation with amonium sulphate and separated on SPE and RP-HPLC and then characterized by tricine electrophoresis and mass spectrometry. The molecular mass of the Ar-AMP was determined to be 4 828.61 Da. The activity of isolated peptides was tested by agar-well diffusion test and the MIC was estimated with the apparatus Bioscreen C. The Ar-AMP exerted antibacterial activity towards E. coli, P. aeruginosa, S. enteritidis and B. megatherium at tested concentration 100 ?g/ml. The. MIC was estimated 50 ?g/ml for E. col

Název v anglickém jazyce

Antibacterial effect of peptide compound isolated from horseradish (Armoracia rusticana) tissue culture

Popis výsledku anglicky

Antimicrobial peptides are an abundant group of proteinaceous compounds widely produced in the plant kingdom. Here, we report the purification and partial characterization of a novel antimicrobial peptide compound from horseradish tissue culture (Armoracia rusticana), named Ar-AMP, with bactericidal activity against both Gram-positive and Gram-negative bacteria. The production of antimicrobial peptides was induced with fungi Aspergillus ochraceus. Ar-AMP was isolated by precipitation with amonium sulphate and separated on SPE and RP-HPLC and then characterized by tricine electrophoresis and mass spectrometry. The molecular mass of the Ar-AMP was determined to be 4 828.61 Da. The activity of isolated peptides was tested by agar-well diffusion test and the MIC was estimated with the apparatus Bioscreen C. The Ar-AMP exerted antibacterial activity towards E. coli, P. aeruginosa, S. enteritidis and B. megatherium at tested concentration 100 ?g/ml. The. MIC was estimated 50 ?g/ml for E. col

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/TA03010877" target="_blank" >TA03010877: Bioremediační a bioaktivní folie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Pharmaceutical, Chemical and Biological Sciences (JPCBS)

ISSN

2348-7658

e-ISSN

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Svazek periodika

3

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

IN - Indická republika

Počet stran výsledku

10

Strana od-do

230-239

Kód UT WoS článku

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EID výsledku v databázi Scopus

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