Occurrence of Fusarium species and mycotoxins in wheat grain collected in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F16%3A43902004" target="_blank" >RIV/60461373:22330/16:43902004 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027006:_____/16:00003565

Výsledek na webu

<a href="http://www.wageningenacademic.com/doi/10.3920/WMJ2015.1917" target="_blank" >http://www.wageningenacademic.com/doi/10.3920/WMJ2015.1917</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3920/WMJ2015.1917" target="_blank" >10.3920/WMJ2015.1917</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Occurrence of Fusarium species and mycotoxins in wheat grain collected in the Czech Republic

Popis výsledku v původním jazyce

Random collections of ears of winter wheat cultivars grown within the Czech Rep. were made during 2004-2013. The ears were assessed for Fusarium head blight symptoms on 0-9 scale and grain samples were analysed for deoxynivalenol (DON) content by enzyme-linked immunosorbent assay. Samples from 2011-2013 were analysed in greater detail, including to detect Fusarium species using polymerase chain reaction assays and determine mycotoxin content (including 'emerging' mycotoxins) using ultra-high performance liquid chromatography coupled with tandem mass spectrometry. The 10-year survey reveals a significant adverse effect from maize as the preceding crop on the accumulation of DON. Most endangered were those areas situated in the eastern part of the country. The study revealed prevailing occurrence of Fusarium poae and Fusarium graminearum within the Czech Rep. The occurrence of F. poae increased greatly in 2012 (with 93.3% of samples infected) in association with relatively warmer and drier weather. These investigations provide abundant evidence as to the presence of so-called 'emerging' mycotoxins, particularly enniatins. The survey also shows that increased content of 'emerging' mycotoxins could be accompanied by high DON content through mixed infections. Among examined toxins, only DON and DON3- glucoside (DON-3G) were positively correlated (r=0.800;P<0.001) while levels of the remaining toxins were independent of one another. DON production by both DON-producing species F. graminearum and Fusarium culmorum was significantly higher in mixed infections with other species. The presence of Fusarium avenaceum with other species appeared markedly to promote the production of 5 out of 6 mycotoxins, namely DON, DON-3G, nivalenol, enniatins, and beauvericin. These five mycotoxins profited also from contamination by F. poae. Moreover, the presence of F. graminearum and F. culmorum appeared to be decisive for zearalenone accumulation, which was not correlated with DON levels.

Název v anglickém jazyce

Occurrence of Fusarium species and mycotoxins in wheat grain collected in the Czech Republic

Popis výsledku anglicky

Random collections of ears of winter wheat cultivars grown within the Czech Rep. were made during 2004-2013. The ears were assessed for Fusarium head blight symptoms on 0-9 scale and grain samples were analysed for deoxynivalenol (DON) content by enzyme-linked immunosorbent assay. Samples from 2011-2013 were analysed in greater detail, including to detect Fusarium species using polymerase chain reaction assays and determine mycotoxin content (including 'emerging' mycotoxins) using ultra-high performance liquid chromatography coupled with tandem mass spectrometry. The 10-year survey reveals a significant adverse effect from maize as the preceding crop on the accumulation of DON. Most endangered were those areas situated in the eastern part of the country. The study revealed prevailing occurrence of Fusarium poae and Fusarium graminearum within the Czech Rep. The occurrence of F. poae increased greatly in 2012 (with 93.3% of samples infected) in association with relatively warmer and drier weather. These investigations provide abundant evidence as to the presence of so-called 'emerging' mycotoxins, particularly enniatins. The survey also shows that increased content of 'emerging' mycotoxins could be accompanied by high DON content through mixed infections. Among examined toxins, only DON and DON3- glucoside (DON-3G) were positively correlated (r=0.800;P<0.001) while levels of the remaining toxins were independent of one another. DON production by both DON-producing species F. graminearum and Fusarium culmorum was significantly higher in mixed infections with other species. The presence of Fusarium avenaceum with other species appeared markedly to promote the production of 5 out of 6 mycotoxins, namely DON, DON-3G, nivalenol, enniatins, and beauvericin. These five mycotoxins profited also from contamination by F. poae. Moreover, the presence of F. graminearum and F. culmorum appeared to be decisive for zearalenone accumulation, which was not correlated with DON levels.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

<a href="/cs/project/QJ1210189" target="_blank" >QJ1210189: Tvorba a identifikace nových zdrojů kombinované odolnosti k významným chorobám a škůdcům pšenice pomocí polních infekčních testů a molekulárních markérů</a><br>

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

WORLD MYCOTOXIN JOURNAL

ISSN

1875-0710

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

317-327

Kód UT WoS článku

000371816400016

EID výsledku v databázi Scopus

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