ASSESSMENT OF CARROTS PRODUCTION SYSTEM USING BIOLOGICALLY ACTIVE COMPOUNDS AND METABOLOMIC FINGERPRINTS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F17%3A43914136" target="_blank" >RIV/60461373:22330/17:43914136 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

ASSESSMENT OF CARROTS PRODUCTION SYSTEM USING BIOLOGICALLY ACTIVE COMPOUNDS AND METABOLOMIC FINGERPRINTS

Popis výsledku v původním jazyce

Carrot (Daucus carota subsp. sativus) is a member of the Umbeliferae family. Carrot contains high quantities of carotenoids, particularly beta-carotene, and is also a good source of dietary fiber, manganese, potassium, ascorbic acid and other vitamins. The aim of this study was an investigation of the influence of farming conditions on the carrot quality. Two carrot varieties, Afalon and Cortina, were grown at locality Troja in the Czech Republic in two different planting densities (600 and 900 pieces per hectare), by organic, integrated, and conventional way of farming. Biologically active compounds in carrots such as carotenoids and ascorbic acid were quantified using LC based techniques with DAD detection and expressed on dry matter content. The higher content of vitamin C was found in variety Cortina. The main carotenoid detected in samples was beta-carotene, but alpha-carotene was also present in significant concentrations. The ratio of alpha- and betacarotene was constant (44:56). Higher carotenoid levels were detected in samples from conventional farming. The effect of mold infestation by Alternaria dauci was investigated as well, the induction of phytoalexine 6-methoxymelein was observed in organic samples. Metabolomic fingerprinting/profiling was used to assess authenticity of carrots. Characterization and classification of carrot samples according to their production system, variety and plant density was carried out using ultra-high performance liquid chromatography coupled to a quadrupole-timeof- flight mass spectrometry (UHPLC?Q-TOF-MS). Metabolomic fingerprints/profiles were obtained in both positive and negative ionisation mode; for chromatographic separation reverse phase and/ or HILLIC analytical column were used. To create robust statistical models, the obtained data were processed by advanced chemometric tools. Examples of PCA and OPLS-DA plots are presented in Figures 1 (way of farming) and 2 (variety and planting density). UHPLC-QTOFMS procedure followed by multivariate data analysis enabled differentiation between organic, integrated, and conventionally produced carrots. Developed statistical models can be also used to distinguish carrot varieties and planting density. Metabolomic data thus were successfully used to predict the production system of the analyzed samples. Additionally, several important marker compounds were tentatively identified.

Název v anglickém jazyce

ASSESSMENT OF CARROTS PRODUCTION SYSTEM USING BIOLOGICALLY ACTIVE COMPOUNDS AND METABOLOMIC FINGERPRINTS

Popis výsledku anglicky

Carrot (Daucus carota subsp. sativus) is a member of the Umbeliferae family. Carrot contains high quantities of carotenoids, particularly beta-carotene, and is also a good source of dietary fiber, manganese, potassium, ascorbic acid and other vitamins. The aim of this study was an investigation of the influence of farming conditions on the carrot quality. Two carrot varieties, Afalon and Cortina, were grown at locality Troja in the Czech Republic in two different planting densities (600 and 900 pieces per hectare), by organic, integrated, and conventional way of farming. Biologically active compounds in carrots such as carotenoids and ascorbic acid were quantified using LC based techniques with DAD detection and expressed on dry matter content. The higher content of vitamin C was found in variety Cortina. The main carotenoid detected in samples was beta-carotene, but alpha-carotene was also present in significant concentrations. The ratio of alpha- and betacarotene was constant (44:56). Higher carotenoid levels were detected in samples from conventional farming. The effect of mold infestation by Alternaria dauci was investigated as well, the induction of phytoalexine 6-methoxymelein was observed in organic samples. Metabolomic fingerprinting/profiling was used to assess authenticity of carrots. Characterization and classification of carrot samples according to their production system, variety and plant density was carried out using ultra-high performance liquid chromatography coupled to a quadrupole-timeof- flight mass spectrometry (UHPLC?Q-TOF-MS). Metabolomic fingerprints/profiles were obtained in both positive and negative ionisation mode; for chromatographic separation reverse phase and/ or HILLIC analytical column were used. To create robust statistical models, the obtained data were processed by advanced chemometric tools. Examples of PCA and OPLS-DA plots are presented in Figures 1 (way of farming) and 2 (variety and planting density). UHPLC-QTOFMS procedure followed by multivariate data analysis enabled differentiation between organic, integrated, and conventionally produced carrots. Developed statistical models can be also used to distinguish carrot varieties and planting density. Metabolomic data thus were successfully used to predict the production system of the analyzed samples. Additionally, several important marker compounds were tentatively identified.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

FOODINTEGRITY 2017 CONFERENCE

ISBN

978-88-941066-5-7

ISSN

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e-ISSN

neuvedeno

Počet stran výsledku

2

Strana od-do

247-248

Název nakladatele

Barilla

Místo vydání

Parma

Místo konání akce

Parma

Datum konání akce

10. 5. 2017

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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