Effect of Agroecological Conditions on Biologically Active Compounds and Metabolome in Carrot

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00547709" target="_blank" >RIV/61388971:_____/21:00547709 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/21:85333 RIV/60461373:22330/21:43922695

Výsledek na webu

<a href="https://www.mdpi.com/2073-4409/10/4/784" target="_blank" >https://www.mdpi.com/2073-4409/10/4/784</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/cells10040784" target="_blank" >10.3390/cells10040784</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of Agroecological Conditions on Biologically Active Compounds and Metabolome in Carrot

Popis výsledku v původním jazyce

Carrot serves as a source of health-beneficial phytochemicals for human diet whose content is affected by agroecological conditions. The effect of conventional, integrated and organic farming on ascorbic acid (AA) and alpha,beta-carotene levels of new carrot cultivars Cortina F1 and Afalon F1 was investigated and their metabolomic profiles were measured by direct analysis in real time ion source coupled with a high-resolution mass spectrometer (DART-HRMS). Cortina and Afalon exhibited high levels of AA and total carotenes under all agroecological conditions tested that fluctuated in broad ranges of 215-539 and 173-456 mg AA.kg(-1) dry biomass and 1069-2165 and 1683-2165 mg carotene.kg(-1) dry biomass, respectively. The ratio of beta- to alpha-carotene in both cultivars was about 1.3. The most important variable for the PCA and the partial least squares discriminant analysis (PLS-DA) models for ethyl acetate extracts measured in positive and negative ionization mode was 6-methoxymellein (6-MM). Total carotene content and 6-MM levels were higher in the organic carrot compared to the conventional one and were correlated with a higher level of spontaneous infection. Other important compounds identified were sitosterol, hexose and various organic acids including antioxidant ferulic and coumaric acids. The findings allow comparison of metabolomic profiles and the AA and carotene contents of both cultivars with those of other commercially used carrots.

Název v anglickém jazyce

Effect of Agroecological Conditions on Biologically Active Compounds and Metabolome in Carrot

Popis výsledku anglicky

Carrot serves as a source of health-beneficial phytochemicals for human diet whose content is affected by agroecological conditions. The effect of conventional, integrated and organic farming on ascorbic acid (AA) and alpha,beta-carotene levels of new carrot cultivars Cortina F1 and Afalon F1 was investigated and their metabolomic profiles were measured by direct analysis in real time ion source coupled with a high-resolution mass spectrometer (DART-HRMS). Cortina and Afalon exhibited high levels of AA and total carotenes under all agroecological conditions tested that fluctuated in broad ranges of 215-539 and 173-456 mg AA.kg(-1) dry biomass and 1069-2165 and 1683-2165 mg carotene.kg(-1) dry biomass, respectively. The ratio of beta- to alpha-carotene in both cultivars was about 1.3. The most important variable for the PCA and the partial least squares discriminant analysis (PLS-DA) models for ethyl acetate extracts measured in positive and negative ionization mode was 6-methoxymellein (6-MM). Total carotene content and 6-MM levels were higher in the organic carrot compared to the conventional one and were correlated with a higher level of spontaneous infection. Other important compounds identified were sitosterol, hexose and various organic acids including antioxidant ferulic and coumaric acids. The findings allow comparison of metabolomic profiles and the AA and carotene contents of both cultivars with those of other commercially used carrots.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cells

ISSN

2073-4409

e-ISSN

2073-4409

Svazek periodika

10

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

18

Strana od-do

784

Kód UT WoS článku

000642878400001

EID výsledku v databázi Scopus

2-s2.0-85105165403