Optimisation of the method for determination of B-complex vitamins in infant formula

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43919076" target="_blank" >RIV/60461373:22330/19:43919076 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Optimisation of the method for determination of B-complex vitamins in infant formula

Popis výsledku v původním jazyce

B-complex vitamins (thiamine (B1), riboflavin (B2), niacin (B3), pantothenic acid (B5), pyridoxine (B6), biotin (B7), folic acid (B9), cobalamin (B12)) represent a group of chemical individuals with different structure, stability, solubility in different solutions and mainly occur in a various amount in food commodities. These hydrophilic vitamins can also occur in various form as a free or bound on proteins, phosphorylated beside that some of them must be quantified as a sum of several substances (e.g. B9 (folic acid and 5-methyl tetrahydrofolate or B6 (pyridoxal, pyridoxine and pyridoxamine)). All these factors should be taken into account during choosing an appropriate analytical method for both isolation and determination of these compounds in food samples including infant formula. The aim of this study was to optimize and validate a method for simultaneous determination of 8 hydrophilic vitamins and their forms in infant formula. Two different sample preparation approaches were tested (with and without enzymatic hydrolysis). For the purification of the obtained extracts solid phase extraction (SPE) was employed. Identification and quantification of target analytes was performed using high performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The performance characteristics of the validated method obtained by the analysis of the SRM 1849a (infant/adult nutritional formula, milk-based) in six replicates, for the procedure without the hydrolysis step, were as follows the recovery 42-103 % (for nine analytes) with repeatability 4-15 % and the limits of quantification 10-25 ng/ml. For three of the target analytes (B1, B9 and B12) the method was not validated.

Název v anglickém jazyce

Optimisation of the method for determination of B-complex vitamins in infant formula

Popis výsledku anglicky

B-complex vitamins (thiamine (B1), riboflavin (B2), niacin (B3), pantothenic acid (B5), pyridoxine (B6), biotin (B7), folic acid (B9), cobalamin (B12)) represent a group of chemical individuals with different structure, stability, solubility in different solutions and mainly occur in a various amount in food commodities. These hydrophilic vitamins can also occur in various form as a free or bound on proteins, phosphorylated beside that some of them must be quantified as a sum of several substances (e.g. B9 (folic acid and 5-methyl tetrahydrofolate or B6 (pyridoxal, pyridoxine and pyridoxamine)). All these factors should be taken into account during choosing an appropriate analytical method for both isolation and determination of these compounds in food samples including infant formula. The aim of this study was to optimize and validate a method for simultaneous determination of 8 hydrophilic vitamins and their forms in infant formula. Two different sample preparation approaches were tested (with and without enzymatic hydrolysis). For the purification of the obtained extracts solid phase extraction (SPE) was employed. Identification and quantification of target analytes was performed using high performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The performance characteristics of the validated method obtained by the analysis of the SRM 1849a (infant/adult nutritional formula, milk-based) in six replicates, for the procedure without the hydrolysis step, were as follows the recovery 42-103 % (for nine analytes) with repeatability 4-15 % and the limits of quantification 10-25 ng/ml. For three of the target analytes (B1, B9 and B12) the method was not validated.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/LM2018100" target="_blank" >LM2018100: Infrastruktura pro propagaci metrologie v potravinářství a výživě v České republice</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů