Selective culture medium for the enumeration of Lactobacillus plantarum in the presence of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43919286" target="_blank" >RIV/60461373:22330/19:43919286 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0023643819307078?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0023643819307078?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.lwt.2019.108365" target="_blank" >10.1016/j.lwt.2019.108365</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Selective culture medium for the enumeration of Lactobacillus plantarum in the presence of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus

Popis výsledku v původním jazyce

Selective enumeration of Lactobacillus plantarum in a mixed culture with yogurt bacteria is difficult, due to their similar growth conditions. We evaluated several culture media to determine their suitability for selective determination. The following media were tested to enumerate the strains of Lactobacillus plantarum, Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus: M17, MRS pH 5.2; 5.6 and 6.2, and modified MRS where glucose was replaced by maltose, sorbitol, mannitol, sucrose or galactose. Further, the addition of vancomycin (20 mg/l) and the use of different cultivation conditions - aerobic or anaerobic, and different cultivation temperatures 30 degrees C, 37 degrees C and 45 degrees C, were chosen to improve selectivity. MRS medium with vancomycin (pH 5.6), and cultivation under aerobic conditions at 30 degrees C was successfully used for selective enumeration of L. plantarum in a mixed culture with yogurt bacteria after cultivation both in broth and in milk. To distinguish between the remaining bacteria, M17 medium under aerobic conditions at 45 degrees C and MRS pH 5.2 under anaerobic conditions under 45 degrees C were recommended for counting of S. thermophilus and L. delbrueckii subsp. bulgaricus, respectively. No statistical differences were found between pour plate and surface plate methods of inoculation (P &gt; 0.05).

Název v anglickém jazyce

Selective culture medium for the enumeration of Lactobacillus plantarum in the presence of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus

Popis výsledku anglicky

Selective enumeration of Lactobacillus plantarum in a mixed culture with yogurt bacteria is difficult, due to their similar growth conditions. We evaluated several culture media to determine their suitability for selective determination. The following media were tested to enumerate the strains of Lactobacillus plantarum, Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus: M17, MRS pH 5.2; 5.6 and 6.2, and modified MRS where glucose was replaced by maltose, sorbitol, mannitol, sucrose or galactose. Further, the addition of vancomycin (20 mg/l) and the use of different cultivation conditions - aerobic or anaerobic, and different cultivation temperatures 30 degrees C, 37 degrees C and 45 degrees C, were chosen to improve selectivity. MRS medium with vancomycin (pH 5.6), and cultivation under aerobic conditions at 30 degrees C was successfully used for selective enumeration of L. plantarum in a mixed culture with yogurt bacteria after cultivation both in broth and in milk. To distinguish between the remaining bacteria, M17 medium under aerobic conditions at 45 degrees C and MRS pH 5.2 under anaerobic conditions under 45 degrees C were recommended for counting of S. thermophilus and L. delbrueckii subsp. bulgaricus, respectively. No statistical differences were found between pour plate and surface plate methods of inoculation (P &gt; 0.05).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21101 - Food and beverages

Projekt

<a href="/cs/project/QK1910036" target="_blank" >QK1910036: Využití potenciálu různých metabolitů divokých kmenů bakterií mléčného kvašení a kvasinek k potlačení výskytu nežádoucích fungálních kontaminantů a prodloužení trvanlivosti mléčných a pekařských výrobků.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

LWT - Food Science and Technology

ISSN

0023-6438

e-ISSN

—

Svazek periodika

114

Číslo periodika v rámci svazku

November 2019, 108365

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

1-7

Kód UT WoS článku

000482245900005

EID výsledku v databázi Scopus

2-s2.0-85068502085