Quality of colostrum as estimated by different methods

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43919933" target="_blank" >RIV/60461373:22330/19:43919933 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.icar.org/wp-content/uploads/2019/12/ICAR-Technical-Series-24-Prague-2019-Proceedings.pdf" target="_blank" >https://www.icar.org/wp-content/uploads/2019/12/ICAR-Technical-Series-24-Prague-2019-Proceedings.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Quality of colostrum as estimated by different methods

Popis výsledku v původním jazyce

Colostrum is initial secretion produced in mammary glands of mammals following parturition. Colostrum is rich in immunoglobulins, lactoferrin, growth factors and many other biologically active substances. Quality of colostrum is important for new-borns, which have immature immune system, moreover colostrum is also widely used as food supplement. The main quality marker of colostrum is concentration of immunoglobulins, in the broad sense content of proteins. The standard method for estimation of immunoglobulin IgG1 is radial immunodiffusion (RID), but this method is lenghty and expensive. Thirty samples of spray dried or lyophilized cow colostrum were analysed by several methods, both rapid screening methods and more precise methods. Measurement of refraction index is rapid and simple method, but correlation with RID is quite week (below 0.8). Three chromatographic methods were tested: size exclusion chromatography (SEC), affinity chromatography (AC) and reverse phase chromatography. Good results were obtained from SEC (R2=0.95), but column is very expensive and it has short lifetime. Similar results gave affinity chromatography, its advantage is short analysis time (8 min). Spectrophotometric methods (Bradford and UV spectroscopy) are not demanding for instrumentation, but the sample preparation is quite complex.

Název v anglickém jazyce

Quality of colostrum as estimated by different methods

Popis výsledku anglicky

Colostrum is initial secretion produced in mammary glands of mammals following parturition. Colostrum is rich in immunoglobulins, lactoferrin, growth factors and many other biologically active substances. Quality of colostrum is important for new-borns, which have immature immune system, moreover colostrum is also widely used as food supplement. The main quality marker of colostrum is concentration of immunoglobulins, in the broad sense content of proteins. The standard method for estimation of immunoglobulin IgG1 is radial immunodiffusion (RID), but this method is lenghty and expensive. Thirty samples of spray dried or lyophilized cow colostrum were analysed by several methods, both rapid screening methods and more precise methods. Measurement of refraction index is rapid and simple method, but correlation with RID is quite week (below 0.8). Three chromatographic methods were tested: size exclusion chromatography (SEC), affinity chromatography (AC) and reverse phase chromatography. Good results were obtained from SEC (R2=0.95), but column is very expensive and it has short lifetime. Similar results gave affinity chromatography, its advantage is short analysis time (8 min). Spectrophotometric methods (Bradford and UV spectroscopy) are not demanding for instrumentation, but the sample preparation is quite complex.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

<a href="/cs/project/QJ1210376" target="_blank" >QJ1210376: Kolostrum jako zdroj nových primárních produktů v potravinách a doplňcích stravy vyznačujících se zlepšenými dietetickými vlastnostmi a vysokým obsahem přirozených biologicky aktivních látek.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the ICAR Conference. ICAR Technical Series No 24 - New traits and adding new value to the recording and ID services in the animal production

ISBN

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ISSN

1563-2504

e-ISSN

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Počet stran výsledku

5

Strana od-do

155-159

Název nakladatele

ICAR

Místo vydání

Rome

Místo konání akce

Prague

Datum konání akce

17. 6. 2019

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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