Enhanced antitumor activity of surface-modified iron oxide nanoparticles and an .alpha.-tocopherol derivative in a rat model of mammary gland carcinosarcoma

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F17%3A00475035" target="_blank" >RIV/61389013:_____/17:00475035 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.2147/IJN.S137574" target="_blank" >http://dx.doi.org/10.2147/IJN.S137574</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2147/IJN.S137574" target="_blank" >10.2147/IJN.S137574</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Enhanced antitumor activity of surface-modified iron oxide nanoparticles and an .alpha.-tocopherol derivative in a rat model of mammary gland carcinosarcoma

Popis výsledku v původním jazyce

Maghemite (.gamma.-Fe2O3) nanoparticles were obtained by coprecipitation of ferrous and ferric salts in an alkaline medium followed by oxidation. The nanoparticles were coated with poly(N,N-dimethylacrylamide) (PDMA) and characterized by transmission electron microscopy, attenuated total reflection (ATR) Fourier transform infrared (FTIR) spectroscopy, dynamic light scattering, thermogravimetric and elemental analyses, and magnetic measurements in terms of particle morphology, size, polydispersity, amount of coating, and magnetization, respectively. The effects of .alpha.-tocopherol (Toc) and its phenolic (Toc-6-OH) and acetate (Toc-6-Ac) derivatives on Fe2+ release from .gamma.-Fe2O3@PDMA, as well as from .gamma.-Fe2O3 and CuFe2O4 nanoparticles (controls), were examined in vitro using 1,10-phenanthroline. The presence of tocopherols enhanced spontaneous Fe2+ release from nanoparticles, with Toc-6-OH exhibiting more activity than neat Toc. All of the nanoparticles tested were found to initiate blood lipid oxidation in a concentration-dependent manner, as determined by analysis of 2-thiobarbituric acid reactive species. Wistar rats with Walker-256 carcinosarcoma (a model of mammary gland carcinosarcoma) received Toc-6-Ac, magnetic nanoparticles, or their combination per os, and the antitumor activity of each treatment was determined in vivo. .gamma.-Fe2O3@PDMA nanoparticles exhibited increased antitumor activity compared to both commercial CuFe2O4 particles and the antitumor drug doxorubicin. Moreover, increased antitumor activity was observed after combined administration of .gamma.-Fe2O3@PDMA nanoparticles and Toc-6-Ac. However, levels of bilirubin, aspartate aminotransferase, and white bloods normalized and did not differ from those of the intact controls. The antitumor activity of the .gamma.-Fe2O3 nanoparticles strongly correlated with Fe2+ release from the nanoparticles but not with nanoparticle-initiated lipid peroxidation in vitro.

Název v anglickém jazyce

Enhanced antitumor activity of surface-modified iron oxide nanoparticles and an .alpha.-tocopherol derivative in a rat model of mammary gland carcinosarcoma

Popis výsledku anglicky

Maghemite (.gamma.-Fe2O3) nanoparticles were obtained by coprecipitation of ferrous and ferric salts in an alkaline medium followed by oxidation. The nanoparticles were coated with poly(N,N-dimethylacrylamide) (PDMA) and characterized by transmission electron microscopy, attenuated total reflection (ATR) Fourier transform infrared (FTIR) spectroscopy, dynamic light scattering, thermogravimetric and elemental analyses, and magnetic measurements in terms of particle morphology, size, polydispersity, amount of coating, and magnetization, respectively. The effects of .alpha.-tocopherol (Toc) and its phenolic (Toc-6-OH) and acetate (Toc-6-Ac) derivatives on Fe2+ release from .gamma.-Fe2O3@PDMA, as well as from .gamma.-Fe2O3 and CuFe2O4 nanoparticles (controls), were examined in vitro using 1,10-phenanthroline. The presence of tocopherols enhanced spontaneous Fe2+ release from nanoparticles, with Toc-6-OH exhibiting more activity than neat Toc. All of the nanoparticles tested were found to initiate blood lipid oxidation in a concentration-dependent manner, as determined by analysis of 2-thiobarbituric acid reactive species. Wistar rats with Walker-256 carcinosarcoma (a model of mammary gland carcinosarcoma) received Toc-6-Ac, magnetic nanoparticles, or their combination per os, and the antitumor activity of each treatment was determined in vivo. .gamma.-Fe2O3@PDMA nanoparticles exhibited increased antitumor activity compared to both commercial CuFe2O4 particles and the antitumor drug doxorubicin. Moreover, increased antitumor activity was observed after combined administration of .gamma.-Fe2O3@PDMA nanoparticles and Toc-6-Ac. However, levels of bilirubin, aspartate aminotransferase, and white bloods normalized and did not differ from those of the intact controls. The antitumor activity of the .gamma.-Fe2O3 nanoparticles strongly correlated with Fe2+ release from the nanoparticles but not with nanoparticle-initiated lipid peroxidation in vitro.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

<a href="/cs/project/GA17-04918S" target="_blank" >GA17-04918S: Léčba glioblastomu pomocí superparamagnetických nanočástic na bázi oxidů železa s povrchově konjugovaným léčivem</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Nanomedicine

ISSN

1178-2013

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

6 June

Stát vydavatele periodika

NZ - Nový Zéland

Počet stran výsledku

12

Strana od-do

4257-4268

Kód UT WoS článku

000402928500002

EID výsledku v databázi Scopus

2-s2.0-85020426594