Abutilon indicum (L.) Sweet Extracts Inhibit Key Glucose Metabolic Enzymes While Enhancing Glucose Transport in L6 Myotubes and 3T3L1 Adipocytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00637641" target="_blank" >RIV/61389030:_____/25:00637641 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/10.1155/jfbc/8252812" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1155/jfbc/8252812</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1155/jfbc/8252812" target="_blank" >10.1155/jfbc/8252812</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Abutilon indicum (L.) Sweet Extracts Inhibit Key Glucose Metabolic Enzymes While Enhancing Glucose Transport in L6 Myotubes and 3T3L1 Adipocytes

Popis výsledku v původním jazyce

Background: Abutilon indicum (L.) Sweet (Malvaceae) is a traditional medicinal plant known for its antidiabetic properties in Ayurveda and other health systems. Aims: This study aims to profile metabolites in Abutilon indicum (L.) Sweet extracts (AI) and elucidate their antidiabetic mechanisms through bioinformatics and experimental methods. Study Design: The ethanolic (AIE) and aqueous (AIA) extracts were evaluated for their inhibitory effects on alpha-glucosidase and alpha-amylase, as well as their impact on glucose metabolism in 3T3-L1 adipocytes and L6 skeletal muscle cells. AIE was characterized via HPLC-DAD-QTOF-MS, with network pharmacology and molecular docking analyses used to explore molecular targets. Methods: In vitro assays were performed to assess enzyme inhibition, and cell line studies HPLC-DAD-QTOF-MS were utilized for compound characterization. Network pharmacology and molecular docking were conducted to reveal underlying antidiabetic mechanisms. Results: LC-MS-QTOF analysis identified gallic acid, stigmasterol, and riboflavin as abundant compounds. The AIE exhibited significant alpha-glucosidase (IC50 = 74.15 +/- 1.61 mu g/mL) and alpha-amylase inhibition (IC50 = 13.41 +/- 0.71 mu g/mL). Moreover, it enhanced glucose consumption in 3T3-L1 cells (IC50 = 6.25 mu g/mL) and promoted glucose uptake in L6 myotubes. Network pharmacology analyses highlighted the PI3K-Akt signaling pathway's role in facilitating glucose transport. Conclusion: The phytochemicals in AIE may contribute significantly to its antidiabetic effects, particularly through the modulation of glucose transport via the PI3K-Akt pathway. Future studies should focus on the preclinical development of safe herbal formulations utilizing these mechanisms for effective diabetes management.

Název v anglickém jazyce

Abutilon indicum (L.) Sweet Extracts Inhibit Key Glucose Metabolic Enzymes While Enhancing Glucose Transport in L6 Myotubes and 3T3L1 Adipocytes

Popis výsledku anglicky

Background: Abutilon indicum (L.) Sweet (Malvaceae) is a traditional medicinal plant known for its antidiabetic properties in Ayurveda and other health systems. Aims: This study aims to profile metabolites in Abutilon indicum (L.) Sweet extracts (AI) and elucidate their antidiabetic mechanisms through bioinformatics and experimental methods. Study Design: The ethanolic (AIE) and aqueous (AIA) extracts were evaluated for their inhibitory effects on alpha-glucosidase and alpha-amylase, as well as their impact on glucose metabolism in 3T3-L1 adipocytes and L6 skeletal muscle cells. AIE was characterized via HPLC-DAD-QTOF-MS, with network pharmacology and molecular docking analyses used to explore molecular targets. Methods: In vitro assays were performed to assess enzyme inhibition, and cell line studies HPLC-DAD-QTOF-MS were utilized for compound characterization. Network pharmacology and molecular docking were conducted to reveal underlying antidiabetic mechanisms. Results: LC-MS-QTOF analysis identified gallic acid, stigmasterol, and riboflavin as abundant compounds. The AIE exhibited significant alpha-glucosidase (IC50 = 74.15 +/- 1.61 mu g/mL) and alpha-amylase inhibition (IC50 = 13.41 +/- 0.71 mu g/mL). Moreover, it enhanced glucose consumption in 3T3-L1 cells (IC50 = 6.25 mu g/mL) and promoted glucose uptake in L6 myotubes. Network pharmacology analyses highlighted the PI3K-Akt signaling pathway's role in facilitating glucose transport. Conclusion: The phytochemicals in AIE may contribute significantly to its antidiabetic effects, particularly through the modulation of glucose transport via the PI3K-Akt pathway. Future studies should focus on the preclinical development of safe herbal formulations utilizing these mechanisms for effective diabetes management.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF FOOD BIOCHEMISTRY

ISSN

0145-8884

e-ISSN

1745-4514

Svazek periodika

2025

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

20

Strana od-do

8252812

Kód UT WoS článku

001504539000001

EID výsledku v databázi Scopus

2-s2.0-105007787515