Near-infrared reflectance calibrations for determining sucrose content in soybean breeding using artificial reference samples

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F12%3A00188047" target="_blank" >RIV/62156489:43210/12:00188047 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Near-infrared reflectance calibrations for determining sucrose content in soybean breeding using artificial reference samples

Popis výsledku v původním jazyce

In food-grade soybeans for human nutrition, sucrose content is a character of interest apart from protein, because sucrose affects flavour and other soy-food properties. As determination of sucrose content through separative or enzymatic methods is not suitable for analysing large numbers of samples, the objective of this research was to develop near-infrared reflectance spectroscopy (NIRS) calibrations for rapid screening of breeding lines for sucrose. Conventional soybean samples have a sucrose content of 30--60 g/kg, whereas high-sucrose reference samples are lacking. Therefore, both natural and artificial (additional sucrose added) reference samples with a sucrose content of up to 140 g/ kg were combined, and a NIRS calibration model was developed(cross-validation R2 = 0.969) for sucrose. Utilizing wavelength ranges with homogeneous performance in both natural and artificial samples, a prediction model was obtained, which suggests a stable performance of the calibration over a wid

Název v anglickém jazyce

Near-infrared reflectance calibrations for determining sucrose content in soybean breeding using artificial reference samples

Popis výsledku anglicky

In food-grade soybeans for human nutrition, sucrose content is a character of interest apart from protein, because sucrose affects flavour and other soy-food properties. As determination of sucrose content through separative or enzymatic methods is not suitable for analysing large numbers of samples, the objective of this research was to develop near-infrared reflectance spectroscopy (NIRS) calibrations for rapid screening of breeding lines for sucrose. Conventional soybean samples have a sucrose content of 30--60 g/kg, whereas high-sucrose reference samples are lacking. Therefore, both natural and artificial (additional sucrose added) reference samples with a sucrose content of up to 140 g/ kg were combined, and a NIRS calibration model was developed(cross-validation R2 = 0.969) for sucrose. Utilizing wavelength ranges with homogeneous performance in both natural and artificial samples, a prediction model was obtained, which suggests a stable performance of the calibration over a wid

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Breeding

ISSN

0179-9541

e-ISSN

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Svazek periodika

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Číslo periodika v rámci svazku

131

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

4

Strana od-do

531-534

Kód UT WoS článku

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EID výsledku v databázi Scopus

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