Development of RT-PCR method for detecting GCLV by specific primers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F16%3A43908792" target="_blank" >RIV/62156489:43510/16:43908792 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.17660/ActaHortic.2016.1110.4" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2016.1110.4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2016.1110.4" target="_blank" >10.17660/ActaHortic.2016.1110.4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development of RT-PCR method for detecting GCLV by specific primers

Popis výsledku v původním jazyce

The method was developed for the detection and resolution of the Garlic common latent virus that causes a serious disease of economically important plants of Allium species, in the Potyvirus presence particularly. GCLV was detected in garlic from Asia, Europe, South America and also from North America. GCLV together with SLV were the most widespread in the collection, as they occurred in 82 and 83% of the accessions in the Czech Republic as was described earlier. GCLV belong to ssRNA positive-strand viruses and is encoded of a single molecule of linear ssRNA of ~8.6 kb, RT-PCR was developed because it is a suitable detection method for RNA viruses. For the development of the RT-PCR method for detecting GCLV two primers were designed which amplify part of genome which encode gene NABP (nucleic acid binding proteins family). These primers amplify a product of approximately 782 base pairs. The product can be easily visualized on agarose gel electrophoresis. RT-PCR conditions were fully optimized for the detection GCLV in infected plants. This is a report on the development of a method for quick and easy, sensitive and economically reasonable virus detection GCLV

Název v anglickém jazyce

Development of RT-PCR method for detecting GCLV by specific primers

Popis výsledku anglicky

The method was developed for the detection and resolution of the Garlic common latent virus that causes a serious disease of economically important plants of Allium species, in the Potyvirus presence particularly. GCLV was detected in garlic from Asia, Europe, South America and also from North America. GCLV together with SLV were the most widespread in the collection, as they occurred in 82 and 83% of the accessions in the Czech Republic as was described earlier. GCLV belong to ssRNA positive-strand viruses and is encoded of a single molecule of linear ssRNA of ~8.6 kb, RT-PCR was developed because it is a suitable detection method for RNA viruses. For the development of the RT-PCR method for detecting GCLV two primers were designed which amplify part of genome which encode gene NABP (nucleic acid binding proteins family). These primers amplify a product of approximately 782 base pairs. The product can be easily visualized on agarose gel electrophoresis. RT-PCR conditions were fully optimized for the detection GCLV in infected plants. This is a report on the development of a method for quick and easy, sensitive and economically reasonable virus detection GCLV

Druh

D - Stať ve sborníku

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/EE2.4.31.0089" target="_blank" >EE2.4.31.0089: Partnerské sítě pro zahradnictví a krajinářskou architekturu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae 1110

ISBN

978-94-6261-104-7

ISSN

0567-7572

e-ISSN

—

Počet stran výsledku

6

Strana od-do

21-26

Název nakladatele

International Society for Horticultural Science

Místo vydání

Leuven

Místo konání akce

Brisbane

Datum konání akce

17. 8. 2014

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000391249900004