Influence of Bacillus spp. enzymes on ultra high temperature-treated milk proteins

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F04%3A00002563" target="_blank" >RIV/62157124:16270/04:00002563 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Influence of Bacillus spp. enzymes on ultra high temperature-treated milk proteins

Popis výsledku v původním jazyce

A model case of long-life half-fat milk contamination with spores of 15 strains of B. licheniformis, B. subtilis a B. cereus isolated from farm environment and from raw milk was used to determine proteolysis by measuring the changes in milk protein contents. The methods of infrared spectroscopy, free tyrosine using Lowry's method according to Juffs and setting of decrease in casein fractions using the SDS-PAGE were employed. Under storage temperature of 4 C no proteolysis was recorded. However, under storage temperature of 24 C the following changes in comparison with initial values were recorded after 3 weeks: a decrease in protein contents from 34.60 g(.)l(-1) to 29.46 - 32.86 g(.)l(-1) and increased free tyrosine contents from 0.65 mg(.)ml(-1) to 2.13 - 1.59 mg(.)ml(-1) depending on Bacillus spp. type. It was detected that milk heating at a temperature of 100degreesC for 10 min and at 135degreesC for 5 s, respectively, the spores of resistant strains Bacillus licheniformis may survi

Název v anglickém jazyce

Influence of Bacillus spp. enzymes on ultra high temperature-treated milk proteins

Popis výsledku anglicky

A model case of long-life half-fat milk contamination with spores of 15 strains of B. licheniformis, B. subtilis a B. cereus isolated from farm environment and from raw milk was used to determine proteolysis by measuring the changes in milk protein contents. The methods of infrared spectroscopy, free tyrosine using Lowry's method according to Juffs and setting of decrease in casein fractions using the SDS-PAGE were employed. Under storage temperature of 4 C no proteolysis was recorded. However, under storage temperature of 24 C the following changes in comparison with initial values were recorded after 3 weeks: a decrease in protein contents from 34.60 g(.)l(-1) to 29.46 - 32.86 g(.)l(-1) and increased free tyrosine contents from 0.65 mg(.)ml(-1) to 2.13 - 1.59 mg(.)ml(-1) depending on Bacillus spp. type. It was detected that milk heating at a temperature of 100degreesC for 10 min and at 135degreesC for 5 s, respectively, the spores of resistant strains Bacillus licheniformis may survi

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta veterinaria Brno

ISSN

0001-7213

e-ISSN

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Svazek periodika

73

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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