Detekce patogenních kmenů Yersinia enterocolitica serotypu O:3 biochemickými, serologickými a PCR metodami

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F07%3A00001150" target="_blank" >RIV/62157124:16270/07:00001150 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Detection of pathogenic Yersinia enterocolitica serotype O:3 by biochemical, serological, and PCR methods

Popis výsledku v původním jazyce

In this study, the pathogenic Y. enterocolitica of serotype O:3 was monitored. The serotype is widely spread in Europe and has been linked to human yersiniosis. For the detection of pathogenic strains were used biochemical and serological methods as wellas PCR methods based on the identification of genes (ail, rfbC, ystA, yadA, virF). The occurrence of Y. enterocolitica O:3 strains was monitored in slaughter animals from a number of farms in the Czech Republic. A total of 3748 samples were collected coming from pigs (1388), cattle (633), poultry (902), and slaughter facilities (825). Fifty-two Y. enterocolitica O:3 isolates were identified by biochemical and serologic methods, and 53 Y. enterocolitica O:3 isolates were identified by PCR methods (46 isolates from pigs, 2 isolates from poultry, 3 isolates from cattle, and 2 isolates from a poultry slaughtering facility). All isolates of Y. enterocolitica O:3 carried genes ail and rfbC, 83% isolates carried gene ystA, 79% isolates carrie

Název v anglickém jazyce

Detection of pathogenic Yersinia enterocolitica serotype O:3 by biochemical, serological, and PCR methods

Popis výsledku anglicky

In this study, the pathogenic Y. enterocolitica of serotype O:3 was monitored. The serotype is widely spread in Europe and has been linked to human yersiniosis. For the detection of pathogenic strains were used biochemical and serological methods as wellas PCR methods based on the identification of genes (ail, rfbC, ystA, yadA, virF). The occurrence of Y. enterocolitica O:3 strains was monitored in slaughter animals from a number of farms in the Czech Republic. A total of 3748 samples were collected coming from pigs (1388), cattle (633), poultry (902), and slaughter facilities (825). Fifty-two Y. enterocolitica O:3 isolates were identified by biochemical and serologic methods, and 53 Y. enterocolitica O:3 isolates were identified by PCR methods (46 isolates from pigs, 2 isolates from poultry, 3 isolates from cattle, and 2 isolates from a poultry slaughtering facility). All isolates of Y. enterocolitica O:3 carried genes ail and rfbC, 83% isolates carried gene ystA, 79% isolates carrie

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA525%2F05%2F0311" target="_blank" >GA525/05/0311: Rychlé metody detekce pathogenních kmenů druhu Yersinia enterocolitica v potravinách</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech Journal of Food Science

ISSN

1212-1800

e-ISSN

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Svazek periodika

25

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

214-220

Kód UT WoS článku

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EID výsledku v databázi Scopus

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