Analysis of DNA for Differentiation of Fish Species in Seafood Samples

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F10%3A00002189" target="_blank" >RIV/62157124:16270/10:00002189 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of DNA for Differentiation of Fish Species in Seafood Samples

Popis výsledku v původním jazyce

Polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) analysis was used to identify fish species and to perform their authentication in commercial seafood products. The mitochondrial cytochrome b gene provides very low intraspecies variability, thus allowing the unequivocal identification of fish species. Universal primers were used for PCR amplification of 464-bp long fragments of this gene. The PCR products were digested with restriction enzymes AluI, HinfI, HaeIII, DdeI, NlaIII, HincII, MboII. Sixty fish samples were obtained from the local markets in the Czech Republic. At 47 samples (78.3%) the results were in agreement with declarations of the producers and 10 samples (16.7%) contained other fish species. There were notgreat differences between fresh fillets (chilled, frozen etc.) and heat processed foodstuffs (tinned, smoked, etc.). Correct designation was at 72.3% and 81.6% samples, respectively. Even if in 3 cases the analysis was unsuccessful the me

Název v anglickém jazyce

Analysis of DNA for Differentiation of Fish Species in Seafood Samples

Popis výsledku anglicky

Polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) analysis was used to identify fish species and to perform their authentication in commercial seafood products. The mitochondrial cytochrome b gene provides very low intraspecies variability, thus allowing the unequivocal identification of fish species. Universal primers were used for PCR amplification of 464-bp long fragments of this gene. The PCR products were digested with restriction enzymes AluI, HinfI, HaeIII, DdeI, NlaIII, HincII, MboII. Sixty fish samples were obtained from the local markets in the Czech Republic. At 47 samples (78.3%) the results were in agreement with declarations of the producers and 10 samples (16.7%) contained other fish species. There were notgreat differences between fresh fillets (chilled, frozen etc.) and heat processed foodstuffs (tinned, smoked, etc.). Correct designation was at 72.3% and 81.6% samples, respectively. Even if in 3 cases the analysis was unsuccessful the me

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Bulletin of the veterinary institute in Pulawy

ISSN

0042-4870

e-ISSN

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Svazek periodika

54

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

5

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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