Characteristics of Escherichia coli from raw vegetables at a retail market in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F13%3A43871932" target="_blank" >RIV/62157124:16270/13:43871932 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027162:_____/13:#0001003

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.ijfoodmicro.2013.09.011" target="_blank" >http://dx.doi.org/10.1016/j.ijfoodmicro.2013.09.011</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijfoodmicro.2013.09.011" target="_blank" >10.1016/j.ijfoodmicro.2013.09.011</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characteristics of Escherichia coli from raw vegetables at a retail market in the Czech Republic

Popis výsledku v původním jazyce

A large epidemic caused by shigatoxigenic Escherichia coli (E. coli) in spring 2011 in Germany resulted in reduction of trust in the health safety of raw vegetables and sprouted seeds. This study focused on the detection and characterization of E. coli in raw vegetables and sprouted seeds sold in the Czech Republic. Out of 91 samples, 24 (26.4%) were positive for the presence of E. coli. Resistance to antimicrobial agents was determined by the disk diffusion method and E-test. Polymerase chain reactionwas used for the detection of selected genes encoding virulence - eaeA, hly, stx1, and stx2 and genes encoding resistance to tetracycline - tetA, tetB, tetC, and tetG and to beta-lactams - blaTEM, blaSHV, and blaCTX. The blaTEM gene was detected in two (7.4 %) isolates and the tetB gene in four (14.8 %) isolates. No hly, stx1, or stx2 genes were present, but the eaeA gene was found in three (11.1 %) isolates from imported vegetables. These isolates can be considered as potentially entero

Název v anglickém jazyce

Characteristics of Escherichia coli from raw vegetables at a retail market in the Czech Republic

Popis výsledku anglicky

A large epidemic caused by shigatoxigenic Escherichia coli (E. coli) in spring 2011 in Germany resulted in reduction of trust in the health safety of raw vegetables and sprouted seeds. This study focused on the detection and characterization of E. coli in raw vegetables and sprouted seeds sold in the Czech Republic. Out of 91 samples, 24 (26.4%) were positive for the presence of E. coli. Resistance to antimicrobial agents was determined by the disk diffusion method and E-test. Polymerase chain reactionwas used for the detection of selected genes encoding virulence - eaeA, hly, stx1, and stx2 and genes encoding resistance to tetracycline - tetA, tetB, tetC, and tetG and to beta-lactams - blaTEM, blaSHV, and blaCTX. The blaTEM gene was detected in two (7.4 %) isolates and the tetB gene in four (14.8 %) isolates. No hly, stx1, or stx2 genes were present, but the eaeA gene was found in three (11.1 %) isolates from imported vegetables. These isolates can be considered as potentially entero

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

<a href="/cs/project/ED0006%2F01%2F01" target="_blank" >ED0006/01/01: Centrum pro aplikovanou mikrobiologii a imunologii ve veterinární medicíne</a><br>

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Food Microbiology

ISSN

0168-1605

e-ISSN

—

Svazek periodika

167

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

196-201

Kód UT WoS článku

000327109500011

EID výsledku v databázi Scopus

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