A study of changes in the concentrations of tetracycline antibiotics in honey

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F16%3A43876151" target="_blank" >RIV/62157124:16270/16:43876151 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A study of changes in the concentrations of tetracycline antibiotics in honey

Popis výsledku v původním jazyce

This work focused on monitoring the stability of the tetracycline antibiotics oxytetracycline, tetracycline and chlortetracycline during sample processing and on optimising the analytical procedure for the determination of tetracycline antibiotics in honey. Stability was tested on standard solutions of tetracyclines in the most commonly used solvents ? water, methanol and Na2EDTA-McIlvaine buffer (citrate phosphate buffer). Subsequently, the preparation procedure was optimised on model honey samples spiked with tetracycline antibiotics at concentration levels of 1, 2 and 4 mg?l-1 using Na2EDTA-McIlvaine buffr as a solvent. The stability of the tetracycline antibiotics was monitored for thirty days at an interval of 3 times a week. Antibiotics were determined by reversed-phased high-performance liquid chromatography with UV detection. Degradation of a multi component standard of tetracycline antibiotics was 69 ? 85% in water, 28 ? 61% in methanol and 46 ? 53% in the Na2EDTA-McIlvaine buffer. Degradation of a multi component standard of tetracyclines in samples of honey at the selected concentrations was 2 ? 61%.

Název v anglickém jazyce

A study of changes in the concentrations of tetracycline antibiotics in honey

Popis výsledku anglicky

This work focused on monitoring the stability of the tetracycline antibiotics oxytetracycline, tetracycline and chlortetracycline during sample processing and on optimising the analytical procedure for the determination of tetracycline antibiotics in honey. Stability was tested on standard solutions of tetracyclines in the most commonly used solvents ? water, methanol and Na2EDTA-McIlvaine buffer (citrate phosphate buffer). Subsequently, the preparation procedure was optimised on model honey samples spiked with tetracycline antibiotics at concentration levels of 1, 2 and 4 mg?l-1 using Na2EDTA-McIlvaine buffr as a solvent. The stability of the tetracycline antibiotics was monitored for thirty days at an interval of 3 times a week. Antibiotics were determined by reversed-phased high-performance liquid chromatography with UV detection. Degradation of a multi component standard of tetracycline antibiotics was 69 ? 85% in water, 28 ? 61% in methanol and 46 ? 53% in the Na2EDTA-McIlvaine buffer. Degradation of a multi component standard of tetracyclines in samples of honey at the selected concentrations was 2 ? 61%.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

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OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Maso International

ISSN

1805-5281

e-ISSN

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Svazek periodika

6

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

3

Strana od-do

25-27

Kód UT WoS článku

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EID výsledku v databázi Scopus

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