A comparison of the Schade and Phadebas methods for determining diastase activity in honey

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F16%3A43876549" target="_blank" >RIV/62157124:16270/16:43876549 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A comparison of the Schade and Phadebas methods for determining diastase activity in honey

Popis výsledku v původním jazyce

The aim of this work was to assess methods for determining diastase activity in honey. Eight samples of honey from the retail network and 48 blends of honey with syrups (Apifood and Apivital) were tested using the Schade method and the Phadebas enzyme method. One of the eight samples of honey failed to meet the requirements of the Decree No. 76/2003 Coll. Diastase activity values of 11.13 units according to Schade, though 7.61 Schade units according to the Phadebas method, were measured in this sample. The two methods produced different diastase activity values, with the values according to Phadebas being on average 12.48% lower. A statistically significant difference (p &lt; 0.05) was found between the methods. The cause of this difference in the results produced by the methods used was probably enzyme substráte specificity ? the specific properties of the starch used as a substrate. The Schade method can be recommended for testing a small number of samples, while the Phadebas method is more suitable for serial analyses.

Název v anglickém jazyce

A comparison of the Schade and Phadebas methods for determining diastase activity in honey

Popis výsledku anglicky

The aim of this work was to assess methods for determining diastase activity in honey. Eight samples of honey from the retail network and 48 blends of honey with syrups (Apifood and Apivital) were tested using the Schade method and the Phadebas enzyme method. One of the eight samples of honey failed to meet the requirements of the Decree No. 76/2003 Coll. Diastase activity values of 11.13 units according to Schade, though 7.61 Schade units according to the Phadebas method, were measured in this sample. The two methods produced different diastase activity values, with the values according to Phadebas being on average 12.48% lower. A statistically significant difference (p &lt; 0.05) was found between the methods. The cause of this difference in the results produced by the methods used was probably enzyme substráte specificity ? the specific properties of the starch used as a substrate. The Schade method can be recommended for testing a small number of samples, while the Phadebas method is more suitable for serial analyses.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

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OECD FORD obor

40401 - Agricultural biotechnology and food biotechnology

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Maso International

ISSN

1805-5281

e-ISSN

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Svazek periodika

6

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

4

Strana od-do

119-122

Kód UT WoS článku

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EID výsledku v databázi Scopus

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