PCR-RFLP identification of meat from red deer, sika deer, roe deer, fallow deer, mouflon, wild boar, hare and cattle

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F19%3A43877351" target="_blank" >RIV/62157124:16270/19:43877351 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16810/19:43877351

Výsledek na webu

<a href="https://actavet.vfu.cz/media/pdf/actavet_2019088010103.pdf" target="_blank" >https://actavet.vfu.cz/media/pdf/actavet_2019088010103.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb201988010103" target="_blank" >10.2754/avb201988010103</a>

Jazyk výsledku

angličtina

Název v původním jazyce

PCR-RFLP identification of meat from red deer, sika deer, roe deer, fallow deer, mouflon, wild boar, hare and cattle

Popis výsledku v původním jazyce

Meat authentication is currently a key topic in relation to the quality and safety of food of animal origin at all levels of production and the global distribution chain. New polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) based on digestion of PCR products with two restriction enzymes, MboII and AciI, have been developed for the specific identification of raw and heat-processed meat from red deer (Cervus elaphus), sika deer (Cervus nippon), roe deer (Capreolus capreolus), fallow deer (Dama dama), mouflon (Ovis musimon), wild boar (Sus scrofa), hare (Lepus europaeus) and cattle (Bos taurus). The PCR primers were targeted in a well-conserved region of the cytochrome b (CYTB) gene to amplify a 378 bp region of all the analysed species. This simple, rapid and cost-effective method is suitable for identification of the meat of game species and their possible substitution by beef.

Název v anglickém jazyce

PCR-RFLP identification of meat from red deer, sika deer, roe deer, fallow deer, mouflon, wild boar, hare and cattle

Popis výsledku anglicky

Meat authentication is currently a key topic in relation to the quality and safety of food of animal origin at all levels of production and the global distribution chain. New polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) based on digestion of PCR products with two restriction enzymes, MboII and AciI, have been developed for the specific identification of raw and heat-processed meat from red deer (Cervus elaphus), sika deer (Cervus nippon), roe deer (Capreolus capreolus), fallow deer (Dama dama), mouflon (Ovis musimon), wild boar (Sus scrofa), hare (Lepus europaeus) and cattle (Bos taurus). The PCR primers were targeted in a well-conserved region of the cytochrome b (CYTB) gene to amplify a 378 bp region of all the analysed species. This simple, rapid and cost-effective method is suitable for identification of the meat of game species and their possible substitution by beef.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta veterinaria Brno

ISSN

0001-7213

e-ISSN

—

Svazek periodika

88

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

103-111

Kód UT WoS článku

000468126600013

EID výsledku v databázi Scopus

2-s2.0-85065507358