An integrated system of four multiplex qPCR assays for the precise and sensitive identification of animal species in food and feed
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F22%3A43880004" target="_blank" >RIV/62157124:16270/22:43880004 - isvavai.cz</a>
Výsledek na webu
<a href="https://doi.org/10.1016/j.foodcont.2021.108781" target="_blank" >https://doi.org/10.1016/j.foodcont.2021.108781</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.foodcont.2021.108781" target="_blank" >10.1016/j.foodcont.2021.108781</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
An integrated system of four multiplex qPCR assays for the precise and sensitive identification of animal species in food and feed
Popis výsledku v původním jazyce
A system of four multiplexes has been developed and optimized to detect up to 18 animal species simultaneously. The system consists of one quadruplex making it possible to detect the most common species - cattle, pigs, and chicken - and to reveal potential inhibitors. An additional 15 meat species can be proven using another three pentaplexes. The uniqueness of the system lies not only in the multiplexing of many different samples, but also in its high sensitivity making it possible to detect 10 pg/microl of DNA or even less. Targeting specific sequences in genomic DNA instead of mitochondrial DNA represents another exclusivity of the system and further increases its specificity. The application of the system on real samples of pet foods obtained from retail showed non-declared species in up to 60% of samples. This demonstrates the great need for a precise and sensitive system for the control of food/feed adulteration and fraud.
Název v anglickém jazyce
An integrated system of four multiplex qPCR assays for the precise and sensitive identification of animal species in food and feed
Popis výsledku anglicky
A system of four multiplexes has been developed and optimized to detect up to 18 animal species simultaneously. The system consists of one quadruplex making it possible to detect the most common species - cattle, pigs, and chicken - and to reveal potential inhibitors. An additional 15 meat species can be proven using another three pentaplexes. The uniqueness of the system lies not only in the multiplexing of many different samples, but also in its high sensitivity making it possible to detect 10 pg/microl of DNA or even less. Targeting specific sequences in genomic DNA instead of mitochondrial DNA represents another exclusivity of the system and further increases its specificity. The application of the system on real samples of pet foods obtained from retail showed non-declared species in up to 60% of samples. This demonstrates the great need for a precise and sensitive system for the control of food/feed adulteration and fraud.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
40301 - Veterinary science
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Food Control
ISSN
0956-7135
e-ISSN
1873-7129
Svazek periodika
135
Číslo periodika v rámci svazku
May
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
8
Strana od-do
nestrankovano
Kód UT WoS článku
000742851800013
EID výsledku v databázi Scopus
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