Development of Indirect Sandwich ELLA for Detection of Insects in Food

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F24%3A43881406" target="_blank" >RIV/62157124:16270/24:43881406 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/24:98681 RIV/60461373:22330/24:43930885

Výsledek na webu

<a href="https://www.mdpi.com/2076-3417/14/23/10794/pdf?version=1732195718" target="_blank" >https://www.mdpi.com/2076-3417/14/23/10794/pdf?version=1732195718</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/app142310794" target="_blank" >10.3390/app142310794</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development of Indirect Sandwich ELLA for Detection of Insects in Food

Popis výsledku v původním jazyce

Edible insects have been officially classified as food in the European Union since 2015. Currently, four insect species are approved for food use. However, no official method for detecting insects in food has been recognized to date. To establish a simple analytical method for insect detection in food, we developed an indirect sandwich (enzyme-linked lectin sorbent assay) ELLA specific for N-acetylglucosamine in chitin and chitosan polymers. The validation of the method demonstrated that the ELLA developed in this study is reliable for insect detection. The limit of detection (LOD) and quantification (LOQ) were 0.006 and 0.028 mg/mL, respectively. Intra-day precision ranged from 2.45% to 30.29%, and inter-day precision from 0.36% to 12.87%. Significant differences in the total amount of chitin and chitosan were observed among the insect products, processed insect products, and samples without any insect addition (p &lt; 0.05).

Název v anglickém jazyce

Development of Indirect Sandwich ELLA for Detection of Insects in Food

Popis výsledku anglicky

Edible insects have been officially classified as food in the European Union since 2015. Currently, four insect species are approved for food use. However, no official method for detecting insects in food has been recognized to date. To establish a simple analytical method for insect detection in food, we developed an indirect sandwich (enzyme-linked lectin sorbent assay) ELLA specific for N-acetylglucosamine in chitin and chitosan polymers. The validation of the method demonstrated that the ELLA developed in this study is reliable for insect detection. The limit of detection (LOD) and quantification (LOQ) were 0.006 and 0.028 mg/mL, respectively. Intra-day precision ranged from 2.45% to 30.29%, and inter-day precision from 0.36% to 12.87%. Significant differences in the total amount of chitin and chitosan were observed among the insect products, processed insect products, and samples without any insect addition (p &lt; 0.05).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

21101 - Food and beverages

Projekt

<a href="/cs/project/QK23020101" target="_blank" >QK23020101: Komplexní laboratorní strategie pro identifikaci druhů hmyzu určeného k lidské spotřebě a produkci zpracované živočišné bílkoviny, autentikace potravin na jeho bázi</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied Sciences-Basel

ISSN

2076-3417

e-ISSN

2076-3417

Svazek periodika

14

Číslo periodika v rámci svazku

23

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

11

Strana od-do

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Kód UT WoS článku

001376873800001

EID výsledku v databázi Scopus

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