Surpassing the Diffraction Limit in Label-Free Optical Microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F24%3A00599726" target="_blank" >RIV/67985882:_____/24:00599726 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22340/24:43929797

Výsledek na webu

<a href="https://pubs.acs.org/doi/10.1021/acsphotonics.4c00745" target="_blank" >https://pubs.acs.org/doi/10.1021/acsphotonics.4c00745</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acsphotonics.4c00745" target="_blank" >10.1021/acsphotonics.4c00745</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Surpassing the Diffraction Limit in Label-Free Optical Microscopy

Popis výsledku v původním jazyce

This perspective article outlines our vision for future directions in label-free super-resolution microscopy. Drawing insights from the success of fluorescence-based super-resolution techniques, we identify parallels in their label-free counterparts and highlight key challenges in the field. We argue here that the progress in optical instrumentation and advanced microscopy methods achieving single-molecule sensitivity, subdiffractional resolution, and high temporal resolution without labels enables observing processes that are either too complex, too fast, or simply not compatible with the labeling. Leveraging a comprehensive review as a foundation, we extrapolate potential developments and, inspired by our recent work, introduce the concept of iSCAT-PAINT combining interferometric scattering microscopy with the concept of PAINT microscopy. This approach resolves fluctuations of interacting single molecules to reveal subdiffractional details of biological structures

Název v anglickém jazyce

Surpassing the Diffraction Limit in Label-Free Optical Microscopy

Popis výsledku anglicky

This perspective article outlines our vision for future directions in label-free super-resolution microscopy. Drawing insights from the success of fluorescence-based super-resolution techniques, we identify parallels in their label-free counterparts and highlight key challenges in the field. We argue here that the progress in optical instrumentation and advanced microscopy methods achieving single-molecule sensitivity, subdiffractional resolution, and high temporal resolution without labels enables observing processes that are either too complex, too fast, or simply not compatible with the labeling. Leveraging a comprehensive review as a foundation, we extrapolate potential developments and, inspired by our recent work, introduce the concept of iSCAT-PAINT combining interferometric scattering microscopy with the concept of PAINT microscopy. This approach resolves fluctuations of interacting single molecules to reveal subdiffractional details of biological structures

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ACS Photonics

ISSN

2330-4022

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

15

Strana od-do

3907-3921

Kód UT WoS článku

001300236200001

EID výsledku v databázi Scopus

2-s2.0-85202491241