Employment of laser induced fusion of living cells for the study of spatial structure of chromatin.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F03%3A12030097" target="_blank" >RIV/68081731:_____/03:12030097 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Employment of laser induced fusion of living cells for the study of spatial structure of chromatin.

Popis výsledku v původním jazyce

We study the transfer of the cell nucleus and individual chromosomes from one living cell to the other one during their fusion. To achieve this, the nuclei of the two fused cells are stained with different fluorescent dyes which serve as identification markers. The fusion itself is done in an inverted optical microscope by combined system that uses optical tweezers to bring two living cells into contact and optical scalpel to punctuate their membranes at the contact point. This process initiates a fusion of both cells into one hybrid cell containing two nuclei. If the fusion product is viable, these nuclei tend to mix together. The dynamics of the fusion process is then visualized by exciting the fluorescently labeled fusion product with a suitable light source. The time evolution of the mutual position of the fused cell nuclei and their final orientation is traced from a video record of the experiment.

Název v anglickém jazyce

Employment of laser induced fusion of living cells for the study of spatial structure of chromatin.

Popis výsledku anglicky

We study the transfer of the cell nucleus and individual chromosomes from one living cell to the other one during their fusion. To achieve this, the nuclei of the two fused cells are stained with different fluorescent dyes which serve as identification markers. The fusion itself is done in an inverted optical microscope by combined system that uses optical tweezers to bring two living cells into contact and optical scalpel to punctuate their membranes at the contact point. This process initiates a fusion of both cells into one hybrid cell containing two nuclei. If the fusion product is viable, these nuclei tend to mix together. The dynamics of the fusion process is then visualized by exciting the fluorescently labeled fusion product with a suitable light source. The time evolution of the mutual position of the fused cell nuclei and their final orientation is traced from a video record of the experiment.

Druh

D - Stať ve sborníku

CEP obor

BH - Optika, masery a lasery

OECD FORD obor

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Projekt

<a href="/cs/project/IAA1065203" target="_blank" >IAA1065203: Využití kombinace laserových mikrosvazkových a cytometrických technik ke studiu struktury a dynamiky lidského genomu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the 13th Polish-Czech-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics (Proc. SPIE 5259).

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

336-340

Název nakladatele

SPIE-The International Society for Optical Engineering

Místo vydání

Washington

Místo konání akce

Krzyzowa [PL]

Datum konání akce

9. 9. 2002

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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