DISCOVERY OF NOVEL AVIAN INTERFERON REGULATORY FACTORS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F23%3A00580686" target="_blank" >RIV/68378050:_____/23:00580686 - isvavai.cz</a>

Výsledek na webu

<a href="http://ccsss.cz/index.php/ccsss/issue/view/41" target="_blank" >http://ccsss.cz/index.php/ccsss/issue/view/41</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

DISCOVERY OF NOVEL AVIAN INTERFERON REGULATORY FACTORS

Popis výsledku v původním jazyce

Interferon regulatory factors (IRFs) are transcription factors that play a key role in anti-viral immunity response of vertebrates. After pathogen recognition by RIG-I-like receptors (RLR), IRF3 and IRF7 activate expression of IFN-β which is together with other type I interferons responsible for expression of interferon-stimulated genes (ISGs) through JAK-STAT1-IRF9 pathway1,2. These pathways are well described in mammals and some other vertebrates but there is little information available in birds. Moreover, IRF9 and IRF3 are thought to be missing in birds4,5even though both of the pathways are functional. Using in silicocomparative analysis and de novo assembly, we were able to annotate IRF9 in multiple avian species across most avian orders, and IRF3 in paleognath birds. We used duck fibroblast cell line as a model system for characterization of avian IRF9. For the first time, we show the role of duck IRF9 (dIRF9) in type I IFN pathway and its role in regulation of ISGs expression after interferon stimulation. Our results suggest time-dependent decrease in the induction of ISGs in the absence of dIRF9 that is partially restored in cells with stable expression of endogenous dIRF9 as well as in cells transiently transfected with endogenous dIRF9. We also focused on a general effect of the dIRF9 absence in cells with or without interferon stimulation using RNAseq data. In summary, we provide the first characterization of previously unknown avian IRF genes

Název v anglickém jazyce

DISCOVERY OF NOVEL AVIAN INTERFERON REGULATORY FACTORS

Popis výsledku anglicky

Interferon regulatory factors (IRFs) are transcription factors that play a key role in anti-viral immunity response of vertebrates. After pathogen recognition by RIG-I-like receptors (RLR), IRF3 and IRF7 activate expression of IFN-β which is together with other type I interferons responsible for expression of interferon-stimulated genes (ISGs) through JAK-STAT1-IRF9 pathway1,2. These pathways are well described in mammals and some other vertebrates but there is little information available in birds. Moreover, IRF9 and IRF3 are thought to be missing in birds4,5even though both of the pathways are functional. Using in silicocomparative analysis and de novo assembly, we were able to annotate IRF9 in multiple avian species across most avian orders, and IRF3 in paleognath birds. We used duck fibroblast cell line as a model system for characterization of avian IRF9. For the first time, we show the role of duck IRF9 (dIRF9) in type I IFN pathway and its role in regulation of ISGs expression after interferon stimulation. Our results suggest time-dependent decrease in the induction of ISGs in the absence of dIRF9 that is partially restored in cells with stable expression of endogenous dIRF9 as well as in cells transiently transfected with endogenous dIRF9. We also focused on a general effect of the dIRF9 absence in cells with or without interferon stimulation using RNAseq data. In summary, we provide the first characterization of previously unknown avian IRF genes

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10607 - Virology

Projekt

<a href="/cs/project/LX22NPO5103" target="_blank" >LX22NPO5103: Národní institut virologie a bakteriologie</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů