The methods of biomedical research in mapping genes that modify immunological parametres and in analysis of gene expression
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F15%3A00300757" target="_blank" >RIV/68407700:21460/15:00300757 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The methods of biomedical research in mapping genes that modify immunological parametres and in analysis of gene expression
Popis výsledku v původním jazyce
Biomedical methods and technology are the fundamental part of biological researches. In the present work we aimed to apply combination of different methods to investigate genetic control of leishmaniasis. Leishmaniasis is a diseases caused by Leishmania parasite. In our lab we use recombinant congenic strain of mice to dissect genes controlling pathology in Leishmania infection. We have analyzed 209 F2 hybrids between strains OcB-43 and O20 and genotyped using 7 markers by PCR on four chromosomes. In order to identify genes controlling response to the diseases we also tested expression of selected genes using Real-time PCR. Data were analyzed using Statistica software. We identified 3 loci controlling size of secondary lymphoid organs. The results of expression analysis showed that expression of selected genes depend on genetic background of the host
Název v anglickém jazyce
The methods of biomedical research in mapping genes that modify immunological parametres and in analysis of gene expression
Popis výsledku anglicky
Biomedical methods and technology are the fundamental part of biological researches. In the present work we aimed to apply combination of different methods to investigate genetic control of leishmaniasis. Leishmaniasis is a diseases caused by Leishmania parasite. In our lab we use recombinant congenic strain of mice to dissect genes controlling pathology in Leishmania infection. We have analyzed 209 F2 hybrids between strains OcB-43 and O20 and genotyped using 7 markers by PCR on four chromosomes. In order to identify genes controlling response to the diseases we also tested expression of selected genes using Real-time PCR. Data were analyzed using Statistica software. We identified 3 loci controlling size of secondary lymphoid organs. The results of expression analysis showed that expression of selected genes depend on genetic background of the host
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EI - Biotechnologie a bionika
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/EE.2.3.20.0092" target="_blank" >EE.2.3.20.0092: Rozvoj výzkumného týmu BIO-OPT-XUV na FBMI ČVUT</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů