Efect of organic acids on bacterium campylobacter jejuni

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Efect of organic acids on bacterium campylobacter jejuni

Popis výsledku v původním jazyce

The aim of the Doctoral thesis was to evaluate the antimicrobial activity of nineteen organic acids and two monoacylglycerols against Campylobacter jejuni. The objective was to determine inhibitory concentrations of organic acids in vitro and to confirmthese results also by in vivo experiment. Two different methods were used to assess the antimicrobial activity of acids: the measurement of cell protein concentration in treated cultures by the biuret method and a SYBR Green based real-time PCR assay. Caprylic, capric and lauric acids were the most efficient antimicrobials among the compounds tested (IC50 <= 0.10 mg/ml). Antimicrobial acitivity of organic acids was pH-dependent, being more pronounced at a lower pH than at a pH higher than 6. The effectof the time of incubation was less evident. Clinical isolate of C. jejuni showed higher tolerance to exposure of organic acids compare to CCM 6214T strain. In the cells treated with capric acid, fumaric acid and monocaprin, the permeabili

Název v anglickém jazyce

Efect of organic acids on bacterium campylobacter jejuni

Popis výsledku anglicky

The aim of the Doctoral thesis was to evaluate the antimicrobial activity of nineteen organic acids and two monoacylglycerols against Campylobacter jejuni. The objective was to determine inhibitory concentrations of organic acids in vitro and to confirmthese results also by in vivo experiment. Two different methods were used to assess the antimicrobial activity of acids: the measurement of cell protein concentration in treated cultures by the biuret method and a SYBR Green based real-time PCR assay. Caprylic, capric and lauric acids were the most efficient antimicrobials among the compounds tested (IC50 <= 0.10 mg/ml). Antimicrobial acitivity of organic acids was pH-dependent, being more pronounced at a lower pH than at a pH higher than 6. The effectof the time of incubation was less evident. Clinical isolate of C. jejuni showed higher tolerance to exposure of organic acids compare to CCM 6214T strain. In the cells treated with capric acid, fumaric acid and monocaprin, the permeabili

Druh

O - Ostatní výsledky

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů