HPLC determination of vitamins B3, B5, and B6 in beef liver

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F09%3A63508764" target="_blank" >RIV/70883521:28110/09:63508764 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

HPLC determination of vitamins B3, B5, and B6 in beef liver

Popis výsledku v původním jazyce

Nicotinamide, D-pantothenic acid and pyridoxine are a water soluble light-sensitive vitamins, that were determined in beef liver by HPLC with UV detection. The separation was carried out on a Supelcosil LC 8 column (4,6 x 150 mm, 5?m), using a mobile phase made up to 90% (0.1 mol.dm-3 KH2PO4, pH 7.0) and 10% HPLC grade methanol. The identification was based on the retention time; the quantification was based on the peak area match against those of standard. The extraction process used in our study was based on a combination of acid (0.1 mol.dm-3 HCI) and enzymatic (2% Clara-diastase suspension) hydrolysis. The nicotinamide and D-pantothenic acid content were 2.98 mg.100g-1, 2.8 mg.100g-1 and the pyridoxine content was 3.9 mg.100g-1 in sample. The proposed HPLC method was successfully applied for the determination of B3, B5 and B6 in beef liver.

Název v anglickém jazyce

HPLC determination of vitamins B3, B5, and B6 in beef liver

Popis výsledku anglicky

Nicotinamide, D-pantothenic acid and pyridoxine are a water soluble light-sensitive vitamins, that were determined in beef liver by HPLC with UV detection. The separation was carried out on a Supelcosil LC 8 column (4,6 x 150 mm, 5?m), using a mobile phase made up to 90% (0.1 mol.dm-3 KH2PO4, pH 7.0) and 10% HPLC grade methanol. The identification was based on the retention time; the quantification was based on the peak area match against those of standard. The extraction process used in our study was based on a combination of acid (0.1 mol.dm-3 HCI) and enzymatic (2% Clara-diastase suspension) hydrolysis. The nicotinamide and D-pantothenic acid content were 2.98 mg.100g-1, 2.8 mg.100g-1 and the pyridoxine content was 3.9 mg.100g-1 in sample. The proposed HPLC method was successfully applied for the determination of B3, B5 and B6 in beef liver.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Fytotechnica et Zootechnica

ISSN

1335-258X

e-ISSN

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Svazek periodika

12

Číslo periodika v rámci svazku

SI

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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